Poroshell 300
Poroshell 300 HPLC Columns from Agilent Technologies
Agilent Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks.
Peptides and proteins are typically separated slowly to reduce the potential peak broadening of these slow diffusing analytes. But Poroshell columns use a unique particle made with a layer of porous silica on a solid core of silica.
This reduces the diffusion distance for proteins making practical rapid HPLC separations of peptides and proteins up to 500 – 1,000 kDA. Poroshell 300SB C18, C8, C3, and 300Extend columns provide excellent stability and selectivity choices with TFA and formic acid mobile faces.
Poroshell 300Extend C18 columns can be used at varying pH levels for unique separations and additional selectivity choices.
All pictures shown are for illustration purpose only. Actual product may vary.
- Buy
- Features
-
- UHPLC separations of biomolecules with superficially porous particles
- 300 Å pore provide high efficiency and recovery with proteins (up to 1,000 kDa) and monoclonal antibodies
- Achieve long lifetime at low pH with Poroshell 300SB; at high pH with 300Extend-C18
- Optimize recovery and selectivity with four different bonded phases – 300SB-C18, 300SB-C8, 300SB-C3, and 300Extend-C18
- Specifications
-
300SB-C18 300SB-C8 300SB-C3 300Extend-C18 Pore Size 300 Å 300 Å 300 Å 300 Å Temp Limits* 90 ºC 90 ºC 90 ºC 40 ºC above pH 8, 60 ºC below pH 8 pH Range 1 - 8 1 - 8 1 - 8 2 - 11 Endcapped No No No Yes USP Designation L1 L7 L56 L1 * 300StableBond columns are designed for optimal use at low pH. At pH 6-8, highest column stability for all silica-based columns is obtained by operating at temperatures <40 °C and using low buffer concentrations in the range of 0.01-0.02 M. At mid or high pH, 300Extend-C18 is recommended.
- Literature
-
-
Agilent BioColumn Selection Brochure
Agilent's high quality BioHPLC columns help you to confidently separate and characterize bio-molecules, including monoclonal antibodies.
-
Agilent Reversed Phase Protein Analysis Brochure
Agilent biocolumns can boost your accuracy and productivity. This document covers complete portfolio of reversed-phase columns for protein identity applications.
-